Diarrhea is one of the principle causes of childhood morbidity and mortality worldwide resulting in 2.5 million deaths annually. Rotavirus infection is the leading cause of severe diarrhea in infants and children under the age of five accounting for 40%-60% of acute gastroenteritis and causing an estimated 500,000 childhood deaths each year. Diagnosis of an infection with rotavirus can be made following diagnosis of gastroenteritis as the cause of severe diarrhea in children. Recently, specific diagnosis of an infection with rotavirus has become available through the detection of virus antigen in stool by immunoassay methods such as latex agglutination assay, EIA, and lateral flow chromatographic immunoassay.

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