Mouse Anti-human IgG Monoclonal Antibody

Human IgG is one of several forms of antibody that are produced by human B cells. Representing approximately 75% of antibodies in human serum, IgG is the most common type of antibody found in the circulation. It is secreted as a monomer with a molecular weight of approximately 150 kDa and forms the Y-like shaped molecule with two antigen binding sites.

IgG antibodies are generated through class switching and maturation in the antibody response and participate predominantly in the secondary immune response.

The detection of pathogen-specific IgG antibodies is a good indication of prolonged microbial infection or reinfection.

B0015 was generated by immunizing mice with human IgG and purified from cell culture via protein A affinity chromatography. It recognizes the Fc portion of human IgG.

  • Physical                                                 Liquid
  • Concentration                  4-6 mg/mL by A280
  • Storage Buffer                           D-PBS, pH 7.2-7.4
  • Preservatives                              0.09% Sodium Azide
  • Purity                                                           ≥ 95% on 12.5% SDS-PAGE
  • Purification                                      Protein A affinity chromatography
  • Application                                      Coating or conjugate antibody in ELISA, ICT, and other immunoassays
  • Cross Reactivity                     No reactivity with human IgM and IgA