Total T4 ELISA CE

The Total Thyroxine (T4) ELISA is a solid-phase enzyme-linked immunosorbent assay based on the principle of the competitive ELISA technique for quantitative determination of Thyroxine in human serum.

The three main kit components are:

1) Solid microwells pre-coated with anti-T4

2) T4 Calibrators

3) Liquid T4 Enzyme Conjugate Mixture comprised of T4 labelled with horseradish peroxidase (T4-HRP)

  • Analytical Sensitivity: 176 µg/dL at 2-SD
  • Specificity: No cross-reactivity or interference with
d-Thyroxine10 µg/mL
d-Triiodothyronine100 µg/mL
Iodotyrosine100 µg/mL
Diiodotyrosine100 µg/mL
TBG50 µg/mL
Human Albumin50 µg/mL
Phenylbutazone25 µg/mL
Phenytoin50 µg/mL
ASA500 µg/mL
Acetaminophen500 µg/mL
  • Accuracy: correlation coefficient 0.98 with a reference method
  • Intra Assay Precision: CV ≤ 5.9%
  • Inter Assay Precision: CV ≤ 7.6%
  • Anti-T4 Ab Coated Microwells
  • Total T4 Calibrators:
    • C1 (0 ng/mL)
    • C2 (2.0 ng/mL)
    • C3 (5.0 ng/mL)
    • C4 (10.0 ng/mL)
    • C5 (15.0 ng/mL)
    • C6 (25.0 ng/mL)
  • Total T4 Assay Control
  • Total T4 Enzyme Concentrate
  • Enzyme Buffer
  • Wash Buffer Concentrate
  • TMB Substrate
  • Stop Solution
  • Product Insert

Catalog #

Product

Quantity

E1025

Total T4 ELISA

96/kit

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