Dengue Serotyping Real-Time PCR Test CE

The Aridia Dengue Serotyping Real-Time PCR Test is designed for the specific and qualitative detection and differentiation of dengue virus (DENV) serotypes 1-4 in serum and plasma, as an aid in the diagnosis of acute DENV infection, alongside all available clinical and epidemiological data, patient history, and other laboratory test outcomes. Dengue serotyping allows for patient and outbreak management in areas endemic to dengue. The product is intended for use by professionals trained in nucleic acid amplification techniques and in vitro diagnostic procedures.

  • Detects and differentiates between the 4 known dengue virus (DENV) serotypes.
  • Comes in a ready-to-use format. All real-time PCR components, including DNA polymerase, reverse transcriptase, primers, probes, and dNTPs, are stabilized in tubes with PCR Mix sufficient to perform 48 RT-PCR tests per kit.
  • All components are stabilized in a lyophilized format to allow ambient shipment and storage (2-40°C).
  • Detection limit of ≤10 RNA copies per reaction for DENV serotypes 1-4
  • 97% of DENV RNA-positive samples were serotyped using the Aridia Dengue Serotyping Real-Time PCR Test, 95% confidence interval (CI) [83%, >99%]

Reagents and Materials provided:

  1. DENV-1 & DENV-4 PCR Mix (White Cap) 2 individually pouched vials (24 reactions per vial)
  2. DENV-2 & DENV-3 PCR Mix (Purple Cap) 2 individually pouched vials (24 reactions per vial)
  3. Dengue Serotyping Positive Control 1 vial in pouch
  4. Dengue Serotyping Negative Control 1 vial in pouch
  5. PCR-Grade Water (1.8 mL) 1 vial in pouch
  6. Instructions for Use

Materials Required but not provided:

  1. Real-Time PCR thermocycler compatible with ROX, HEX (JOE, VIC) and Cy5 channels
  2. RNA purification kit
  3. Sterile RNase-free 1.5 mL centrifuge tubes
  4. PCR tubes/strips/plate and optical caps/film
  5. Centrifuge for 1.5 mL tubes and PCR-well strips or 96-well plate (if available)
  6. Vortex
  7. Micropipettes (0.5-20 µL, 20-200 µL and 1000 µL)
  8. Filter micropipette tips
  9. Gloves and other recommended PPE

 

CATALOG #PRODUCTQUANTITYSPECIMEN
P0061Dengue Serotyping Real-Time PCR Test48/KitSerum/Plasma
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