Dengue Virus Type 2 Envelope Protein

Dengue virus (DENV) is an enveloped, single-stranded, positive-sense RNA virus that includes four related but distinct serotypes (DENV1, 2, 3, and 4). It encodes three structural proteins (capsid, membrane and envelope) and seven non-structural proteins (NS1,-2a, -2b, -3, -4a, -4b and -5).

The envelope (E) glycoprotein mediates virion attachment to the receptor and fusion of the virus envelope with the target cell membrane. The recombinant E protein can be used in diagnostic assays for the detection of either primary or secondary dengue infection, to overcome safety issues associated with the use of whole virus.

A2302 is composed of the full length amino acid sequence of the envelope protein of the dengue virus type 2 which was cloned into an insect expression vector. The secreted and soluble recombinant protein was purified with affinity chromatography.

  • Physical Status                       liquid
  • Concentration                           ≥ 1 mg/mL by Bradford protein assay
  • Storage Buffer                           D-PBS, pH 7.2-7.4
  • Preservatives                              0.099% Thimerosal, 1μg/mL Leupeptin, 1μg/mL Aprotinin, and 1μg/mL Pepstatin A
  • Purity                                                           ≥ 95% on 12.5% SDS-PAGE
  • Purification                                      Affinity chromatography
  • Application                                   ELISA, ICT, and other immunoassays
  • Cross Reactivity                     N/A