Free T3 ELISA CE

The RecombiLISA Free T3 ELISA is a competitive solid-phase enzyme-linked immunosorbent assay.  The two main kit components are:

  1. A 96-well breakable microplate pre-coated with anti-fT3 antibody;
  2. A liquid conjugate solution composed of horseradish peroxidase with T3 (HRP-T3 conjugate)

This test is intended for the quantitative determination of free triiodothyronine (fT3) level in human serum. It is intended to be used by professionals as an aid in the diagnosis of thyroid dysfunction. Any interpretation or use of this test result must also rely on other clinical findings as well as on the professional judgment of health care providers.

Recommended for use:

  • aid in diagnosis of thyroid disease including hyperthyroidism and thyrotoxicosis
  • monitor pregnancy as well as patients undergoing treatment for thyroid disorder, pituitary disease, and thyroid cancer

Quantitative measurement of the more stable fT4/fT3 levels is believed to be more accurate in most cases. If fT3 levels are abnormal, a diagnosis can be made depending on the combination of TSH, fT4, and fT3 levels. For example, low TSH, normal fT4, and low fT3 levels usually indicates hyperthyroidism. Combining TSH with T4/T3 and fT4/fT3 measurements provides more information for the determination of thyroid status.

  • Sensitivity:274 ± 0.077 pg/mL at 95% confidence level
  • Specificity: <0.001 cross reactivity with l-thyroxine at 10 μg/mL, iodotyrosine at 10 μg/mL, diiodotyrosine at 10 μg/mL, and d-thyroxine at 10 μg/mL, respectively
  • Accuracy: correlation coefficient 0.958 ± 0.087 at 95% confidence level
  • Intra Assay Precision: CV < 5.1%
  • Inter Assay Precision: CV < 7.5%
  • Shelf life: 18 months
  • Open kit stability:  8 weeks  at 2-8°C, or until the expiration date
  • Anti-fT3 Ab Coated Microwells
  • fT3 Calibrators C1-C6
  • HRP-T3 Conjugate
  • Wash Buffer (30X Concentrate)
  • TMB Substrate
  • Stop Solution
  • ELISA Working Sheet
  • Product Insert

Catalog #

Product

Quantity

Specimen

E1011

Free Thyroxine (fT3) ELISA Kit

96/kit

Serum

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To learn about distribution opportunities or for additional product information, please contact info@ctkbiotech.com

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