Pan-Plasmodium Aldolase Antigen
Malaria aldolase is a key enzyme that helps convert glucose into energy in malaria parasites, and is critical for intraerythrocytic merozoite lifestage. Aldolase also binds to Actin, Adhesin and Thrombospondin-related anonymous protein (TRAP), suggesting its function during the invasion. Higher vertebrates usually have multiple tissue-specific aldolase isoenzymes. However, P. falciparum and P. vivax have only one aldolase isoenzyme, which
is highly conserved at the amino acid sequence and structural level. Therefore, malaria aldolase is used as one of the markers for diagnosis of malaria infection.
A3001 is composed of the full length amino acid sequence of the P. falciparum aldolase which was cloned into the bacteria expression vector. The soluble recombinant protein was purified from E. coli with affinity chromatography.