Plasmodium Falciparum HRP-II Antigen

Plasmodium falciparum (Pf) is a protozoan parasite, one of the species of Plasmodium that causes the most dangerous form of malaria, with the highest rate of complications and mortality in humans. Transmission occurs through the bite of female Anopheles mosquitos infected with the parasite.

The Histidine-rich protein II (HRPII) is an essential P. falciparum protein and it is expressed during the intra-erythrocytic stages of the parasitic life cycle.

Since HRPII is expressed only by P. falciparum, it can be used as a marker to distinguish between a P. falciparum malaria infection and infection with one of the other four non-falciparum species.

A3000 is composed of the full length amino acid sequence of the P. falciparum HRPII which was cloned into a bacterial expression vector. The soluble recombinant protein was expressed by E. coli and purified with affinity chromatography.

  • Physical Status                       Lyophilized powder
  • Concentration                           ≥ 2 mg/mL by Bradford protein assay
  • Storage Buffer                           10 mM Tris-HCl, pH8.5, 10 mM NaCl
  • Preservatives                              0.099% Thimerosal, 100 μg/ml PMSF, 1 μg/mL Leupeptin, 1 μg/mL Aprotinin and 1 μg/mL Pepstatin A after reconstitute with distilled H2O
  • Purity                                                           ≥ 95% on 12.5% SDS-PAGE
  • Purification                                      Affinity chromatography
  • Application                                   Pf-HRP-II immunoassay control
  • Cross Reactivity                     N/A