Plasmodium Falciparum-LDH Antigen

Plasmodium falciparum (Pf) is a protozoan parasite, one of the species of Plasmodium that causes malaria in humans through the bite of female Anopheles mosquitos infected with the parasite. Malaria caused by this species (also called malignant or falciparum malaria) is the most dangerous form of malaria, with the highest rates of complications and mortality.

Pf-LDH (Plasmodium falciparum specific lactate dehydrogenase enzyme) is an essential Plasmodium protein and it is expressed during the intra-erythrocytic stages of the parasitic life cycle. Pf-LDH is used as a serological diagnostic marker for the detection of P. falciparum malaria.

A3005 is composed of the full length amino acid sequence of the P. falciparum LDH which was cloned into a bacterial expression vector. The soluble recombinant protein was expressed by E. coli and purified with affinity chromatography.

  • Physical Status                       liquid
  • Concentration                           ≥ 1 mg/mL by Bradford protein assay
  • Storage Buffer                           25 mM Tris-HCl, pH8.0, 50 mM NaCl, and 50% Glycerol
  • Preservatives                              0.09% NaN3, 5 mM EDTA, 1μg/mL Leupeptin, 1μg/mL Aprotinin, and  1μg/mL Pepstatin A
  • Purity                                                           ≥ 95% on 12.5% SDS-PAGE
  • Purification                                      Affinity chromatography
  • Application                                   Pf-LDH immunoassay control
  • Cross Reactivity                     N/A